Abstract
ENZYMES INVOLVED IN RECOMBINANT DNA TECHNOLOGY

Dr. Priti Gupta*

ABSTRACT

Recombinant DNA means combining DNA segments from two different sources. In natural system i.e. in vivo conditions, recombination can occur during sexual reproduction in the prophase I of meiosis. But in artificial system i.e. in vitro conditions, recombinant DNA can be produced by using scientific techniques. This synthetically generated DNA can have combinations ranging from human and bacteria for insulin production, yeast and surface antigen of hepatitis virus for hepatitis B vaccine to two plants of different families forming a new transgenic plant. The first recombinant DNA was created by Stanley Cohen, H. Boyer et al in 1973. They have inserted DNA from African clawed frog into bacterium. This article deals with the basic technique of producing recombinant DNA, tools and enzymes used in generating recombinants.

Keywords: RDT, Tools of RDT, Restriction enzymes, typeI, typeII, typeIIs, type III, DNA ligase, Alkaline phosphatase.


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