Abstract
DIARRHOEAL PROTECTIVE ACTIVITY OF HOT AQUEOUS STEM-BARK EXTRACT OF CARPOLOBIA LUTEA IN RATS.

Lucky Legbosi Nwidu*, Moses Bariweni and Iyele Kamenebali

ABSTRACT

The objective is to scrutinize the darrhoeal protective activity of Carpolobia lutea (CL) Hot aqueous stem-bark extract (HASE) in rodents. The diarrhoeal protective effect of CL HASE (12.5-50 mg/kg p.o.) was scrutinize using experimental models of castor-oil induced diarrhoea in wistar rats and its effect compared to standard drugs. CL HASE (12.5-50 mg/kg p.o.) produced a dose-dependent decrease in normal and castor oil-induced intestinal transit, castor oil- induced diarrhoea and enteropooling compared to the vehicle treated group. The 25.0 mg/kg CL HASE significantly reduced (p< 0.05 - 0.001) the number, total number, the weight and total weight of stool from the control values of 7.83± 0.42 , 10.50 ± 1.09, 3.83 ± 0.42 and 5.85 to 0.0, 1.50 ± 0.43, 0.0 and 0.81 ± 0.03 respectively. It reduces intestinal fluid by 58.79 % and weight by 58.6% compared to morphine (5 mg/kg s.c.) of 24.73% and 46.04 % inhibition respectively. It reduces diarrhoea scores of 27.46 ± 4.00 to 1.67 ± 0.76 in a dose dependent significant manner (p < 0.001). The protection of 93.92% by 25.0 mg/kg CL HASE was reversed significantly (p < 0.001) by isosorbide dinitrate (150 mg/kg p.o.), yohimbine (1 mg/kg s.c.) and diphenoxylate (0.5 mg/kg p.o.) to 91.51 %, 60.67 % and 87.84 % respectively. Preliminary phytochemical screening reveal the presence of saponins, alkaloids, cardiac glycosides and tannins. In the acute toxicity study, no mortality was observed orally up to 5 000 mg/kg in rats, but an LD50 of 125.05 mg/kg i.p. in mice. The study indicate that CL HASE possess antidiarrhoeal activity mediated via the stimulation of mucosal α-adrenergic receptors and inhibition of nitric oxide pathway.

Keywords: Diarrhoeal protective effect, ethanolic stembark extract, Carpolobia lutea.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • OCTOBER ISSUE PUBLISHED

    OCTOBER 2018 Issue has been successfully launched on 1 October 2018

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 4.382 to 4.918 due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT