Abstract
SPECTROPHOTOMETRIC AND STABILITY-INDICATING SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF LORNOXICAM IN PURE FORM AND PHARMACEUTICAL PREPARATION.

Hamed Hamed Mohamed Abou-Seada, Khalid Abdel-Salam Attia, Mohammed Wafaa Nassar, Mohamed Saleh Emara*

ABSTRACT

Four Simple, rapid, sensitive, accurate and precise methods were developed for the determination of lornoxicam in bulk powder , in pharmaceutical preparation and in presence of its degradate.Method (A) ratio difference method ;is based on measuring the difference in the amplitude of intact lornoxicam in presence of its degradation product at two different wavelengths ,this is done at 233.8 nm and 249.4 nm in the range of 5 – 35 μg ml-1 with LOD of 0.136 μg ml-1 and LOQ of 0.453 μg ml-1.Method (B) mean centering method; the method was applied for the analysis of lornoxicam in presence of its acidic degradation product this is done at 243.8 nm in the range of 5 – 35 μg ml-1 with LOD of 0.072 μg ml-1 and LOQ of 0.241 μg ml-1.Method (C) o-phenanthroline-Fe (III) mixture; upon reacting o-phenanthroline-Fe (III) with lornoxicam, Fe (III) is reduced to Fe (II) which form a red complex with O-phenanthroline this is done at 512 nm in the range of 0.8 – 5.6 μg ml-1 with LOD of 0.010 μg ml-1 and LOQ of 0.034 μg ml-1.Method (D) oxidative coupling with 3-methylbenzothiazoline-2-one hydrazone (MBTH);the reaction of lornoxicam with MBTH in presence of oxidizing agent (cerric ammonium sulphate) proceeds via oxidative coupling to form an orange colored product measured at 456 nm in the range of 3-18 μg ml-1 with LOD of 0.199 μg ml-1 and LOQ of 0.664 μgml-1.The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision.

Keywords: Lornoxicam, Ratio difference, Mean centering, O-phenanthroline, MBTH.


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