Abstract
CARBAPENEM RESISTANCE-DETERMINING GENES AMONG MULTI-DRUG RESISTANT BACTERIAL ISOLATED FROM CLINICAL SAMPLES IN BASRAH GOVERNORATE

Ismaal Jmia Abas and Maytham A. Al-Hamdani*

ABSTRACT

Increasing resistance to carbapenems, may significantly reduce the choice of effective antibiotics. This study was conducted to determine the occurrence of carbapenmase producing bacteria isolates obtained from Basrah hospitals. Isolates were identified. And Carbapenem susceptibility was assayed by using VITEK 2 system. Phenotypic detection of carbapenemase was performed using four method Combined disc test, modified Hodg tests, RAPIDEC CARBA NP test, ChromID Carba media. Then isolates were subjected to monoplex PCR targeting blaNDM-1 and OXA-48 genes. 61(20.74%) Carbapenem Resist gram negative Bacteria isolates were recovered from clinical samples. 61 (100%) of isolate was found to be imipenem resistant. 31 (70.45%) of isolates give positive result to Combined disc test from 44 isolates which give positive rustle in PCR method whereas 35(79.54%) isolates showed positive results with modified Hodg test, 41(93.18%) of isolates give positive result to ChromIDCarba media, while 44(97.72%) of isolates give positive result to RAPIDEC CARBA NP test .PCR experiments showed 29 (47.54%)) isolates were harbored blaNDM-1gene and 28(45.90%) isolates were harbored OXA-48gene.

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