Abstract
FREQUENCIES OF GSTP1( Ile105Val) POLYMORPHISM AND ITS ASSOCIATION WITH CML IN SUDANESE PATIENTS

Elharam Ibrahim Abd allah, Abdel Rahim Mahmoud Muddathir*, Salah Eldin G. Elzaki, Wala Eldin Osman Elradi and Ream Elzain Abdelgadir

ABSTRACT

Background: Glutathione S-transferases (GSTs) are enzymes belong to phase II metabolic isozymes, best known for their ability of detoxification of xenobiotic. Several studies reported that gene Polymorphisms within GSTs family conferred a significant association with increased risk of CML. There is no published data about the association of gene polymorphism of these enzymes to CML Sudanese patient’s, So the present study aimed to find out if there was any association between GSTP1 gene polymorphism and CML in Sudanese patients. Materials and methods: This was case control study conducted between January 2016 and June 2017 in which a total of 115 patients with chronic myeloid leukaemia attended the Radiation and Isotope Centre-Khartoum and 104 un related apparently healthy individual as control group were involved. DNA extracted from collected EDTA venous blood samples and analysed by RFLP (Restriction fragments length polymorphism) to detect the presence of GSTP1 (Il105Val). Results: A total of 58.2% (67) of CML patients had heterozygous (Ile/Val) type of GSTP1 Ile105Val polymorphism, 4.35% (5) patients had the homozygous type (Val/ Val) and 37.3% (43) had the wild genotype of GSTP1 (Ile / Ile). In contrast there were 40.3% (42) of the control group with heterozygous (Ile/Val) type, 1.9% (2) with homozygous type (Val/ Val) and 57.6% (60) with wild genotype of GSTP1 (Ile / Ile). GSTP1 Ile105Val polymorphism frequency was found to be significantly elevated in patient with CML 62.6% (72) compared to control group 42.3% (44) (OR = 2.28, 95% confidence interval (CI) 1.33–3.91, p value = 0.002). Discussion and Conclusion: GSTP1 Ile105Val polymorphism was found to be significantly elevated in patient with CML and were associated with CML development and progression, also CML patients in advanced phase (accelerated and blast crisis) had higher frequency of GSTP1 Ile105Val polymorphism compared to chronic phase and this was agreed with most of the published studies.

Keywords: GSTP1: Glutathione S-transferase Pi, Ile105Val: Iso Lucin 105 Valin, CML: Chronic Myeloid Leukaemia.


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