Abstract
IN VITRO MICROPROPAGATION OF BLACK GRAM (VIGNA MUNGO L) THROUGH SHOOT TIP EXPLANTS INDUCED BY THIDIAZURON (TDZ)

Srilatha Thallapally*

ABSTRACT

The effect of Thidiazuron (TDZ) was investigated on in vitro shoot proliferation from shoot tip explants of Black gram (Vigna mungo.L). Murashige and Skoog (MS) medium containing TDZ (1.0–8.0 mg/L) was effective in inducing shoot buds and maintaining high rates of shoot multiplication on hormone free medium. The highest shoot regeneration frequency (78%) and mean number (06.0 ± 0.43) of shoots per explants were achieved from shoot tip explants segments cultured on MS medium supplemented with (6.0mg/L mg/L) TDZ for 4 weeks prior to transfer to MS medium without TDZ for 8 weeks. The elongated shoots were transferred to Indole Butyric Acid (IBA) (1.0mg/L–5.0 mg/L) for root induction. Rooting was observed within two weeks of culture. The regenerated shoots rooted best on MS medium containing (3.0 mg/L) indole-3-butyric acid (IBA). Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions.

Keywords: In Vitro Micropropagation, Shoot Tip Explants, Black Gram (Vigna mungo.L).


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