Abstract
EFFICIENT CALLUS INDUCTION AND INDIRECT PLANT LET PROLIFERATION FROM COTYLEDONARY EXPLANTS OF GYMNEMA SYLVESTRE (RETZ) R. BR. EX ROEMER & SCHULTES. AN IMPORTANT MEDICINAL PLANT

Anitha Devi Uppu*

ABSTRACT

Gymnema sylvestre Retz. R.Br. Ex Roemer & Schultes, commonly called as Gudmar” (gud -jaggery, mar-kills) a medicinally important branched woody climber, distributed throughout India in dry forest up to 600 m. belongs to the Asclepiadaceae family commenced 100 years ago, has been employed to control diabetes by traditional medicinal practitioners of India for several centuries. Nowadays focus is being placed on Gymnema sylvestre callus induction and plantlet proliferation. In this study the Cotyledonary explants of two weeks old seedlings of Gymnema sylvestre were induced for callus induction on MS media containing different combinations and concentrations of growth regulators. Different callusing media containing varying levels of 2.4-D/IAA/NAA (1.0- 5.0mg/L) were tested for callus induction response. Maximum (90%) callusing response was obtained from Cotyledonary explants on MS medium containing (3.0mg/L) NAA. After 8 weeks of induction, the calluses were transferred on different regenerated media containing varying level of BAP/Kn/TDZ (1.0-5.0mg/L). Maximum shoot bud differentiation from callus culture was achieved on MS Medium fortified with TDZ (3.0mg/L). Elongation and further development of shoot buds into shoots were achieved on MS medium supplemented with TDZ (3.0mg/L). This is the first report of in vitro callus induction and plantlet regeneration in Gymnema sylvestre. The regenerated elongated shoots were transferred to Indole Butyric Acid (IBA) (1.0mg/L–5.0mg/L) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 86%. Plants looked healthy with no visually detectable phenotypic variations.

Keywords: Callus Induction, Plant let Proliferation Indirect Regeneration, Cotyledonary Explants and Gymnema Sylvestre.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • EJBPS NEW ISSUE PUBLISHED

    Its Our pleasure to inform you that, EJBPS November 2017 Issue has been Published, Kindly check it on http://www.ejbps.com/ejbps/current_issues

  • NOVEMBER ISSUE PUBLISHED

    NOVEMBER 2017 Issue has been successfully launched on 1 November 2017

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 45.77, due to High Quality Publication in EJBPS at International Level

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 3.881 to 4.382 due to high quality Publication at International Level.

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT