Abstract
DIVERSITY OF NON-SYMBIOTIC NITROGEN FIXING BACTERIA FROM SOILS OF NORTH GUJARAT, INDIA

*P. Patel and S. Bhatt

ABSTRACT

Diversity of non-symbiotic nitrogen fixing bacteria isolated from four districts of North Gujarat; Mehsana, Sabarkantha, Banaskantha and Patan have been investigated. Non-Symbiotic nitrogen fixing bacteria were randomly selected for studying their Morphological and Physiological characterization, Biochemical Characterization, Genetic analysis for the identification up to species level, and Phylogenetic relatedness of the isolates. Total Fifty-nine strains of non-symbiotic nitrogen fixing bacteria were isolated from diverse agro-ecological locations of North Gujarat. DNA characterization by using Amplified ribosomal DNA restriction analysis could separate these bacteria into 16 different groups. They are found to be Azotobacter salinestris, Azotobacter tropicalis, Azotobacter vinelandii, Nocardiodies nitrophenolicus, Streptomyces thermocarboxydovorans, Agrobacterium tumifaciens, one isolate similar with uncultured Sp., Variovorax soil strain, Mycobacterium cosmeticum, Enterobacter ludwigi, Rhodococcus corynebacterioides, Sinorhizobium saheli, Bacillus niabensis, Planococcus sp., Microbacterium sp., and Arthrobacter sp. The genetic analysis showed that non-symbiotic nitrogen fixing bacteria are highly diverse in nature and cannot specific to any each ecosystem. Our result indicates to explore the Biotechnological applications in different fields of environmental microbiology along with their nitrogen fixing capacity.

Keywords: Nitrogen Fixing Bacteria, Amplified ribosomal DNA restriction,16S r-RNA, Diversity, Soil.


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