Abstract
CRYOPRESERVATION OF VANILLA POLLEN AND IT’S UTILIZATION IN INTER SPECIFIC HYBRIDIZATION BETWEEN V. PLANIFOLIA AND V. APHYLLA.

Minoo Divakaran, K. Nirmal Babu* and K. V. Peter

ABSTRACT

Vanilla from, Vanilla planifolia which is native to Central America, is an important spice and plays a major role in global economy. Improvement in Vanilla planifolia, is hampered by narrow gene pool in the crop. A few related species occur with useful characters like natural seed set and resistance to fungal diseases. Combining the available gene pool in the genus will help in broadening the genetic base and converging the useful genes into cultivated vanilla from wild species. Interspecific hybridization requires synchronized flowering between the species and or availability of viable pollen. Pollen collected from flowers showed maximum viability (89.4%) on the day of anthesis and that they were 80.1% viable almost 20h prior to anthesis too. Pollen from two asynchronously flowering species of Vanilla viz., cultivated V. planifolia and its wild relative V. aphylla, were cryopreserved after desiccation to % moisture, pretreated with cryoprotectant Dimethyl sulphoxide (5%) and cryopreserved -196ºC in Liquid Nitrogen. This cryopreserved pollen was latter thawed and tested for their viability both in vitro and in vivo. A germination percentage of 82.1% and 75.4% in V. planifolia and V.aphylla pollen respectively were observed indicating their viability. This cryopreserved pollen of V. planifolia was used successfully to pollinate V.aphylla flowers resulting in fruit set. the seeds thus obtaines were sussfully cultured to develop hybrid plantlets. This system is of great importance and can be used for conserving the haploid gene pool of Vanilla in cryobanks and their subsequent utility in crop improvement.

Keywords: Cryopreservation, hybridization, germplasm conservation, pollen, Vanilla aphylla, V.planifolia.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • EJBPS NEW ISSUE PUBLISHED

    Its Our pleasure to inform you that, EJBPS November 2017 Issue has been Published, Kindly check it on http://www.ejbps.com/ejbps/current_issues

  • NOVEMBER ISSUE PUBLISHED

    NOVEMBER 2017 Issue has been successfully launched on 1 November 2017

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 45.77, due to High Quality Publication in EJBPS at International Level

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 3.881 to 4.382 due to high quality Publication at International Level.

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT