Abstract
METHOD DEVELOPMENT AND VALIDATION OF AN ANTI-HYPERTENSIVE AGENT BY USING RP-HPLC

Badikela Ramakrishna*, Kondapuram Parameshwar, Donapati Jalander and Kosika Sandeep

ABSTRACT

A new Simple, rapid and sensitive isocratic RP-HPLC method has been developed and validated for the determination of Atenolol in tablet formulation. The method employs SHIMADZU HPLC system on ODS, C18 column (250*4.6mm and 5 μm). Best chromatographic separation was achieved by using 30mm Ammonium acetate (pH 3): acetonitrile: methanol in the ratio of 50:30:20 as mobile phase with a flow rate of 1.0mL/min and isocratic elution with a total runtime of 5 minutes. Detection of the compound was carried out at 226nm. The retention time of Atenolol was found to be 2.527. The linearity studies ranges from at the concentration range of 60-140μg/ml. This method was validated for accuracy, precision, linearity, ruggedness and robustness as per ICH guidelines. The present newly developed method was found to be accurate, precise and can be useful for routine Quality control analysis.

Keywords: Atenolol, HPLC, ODS, ICH.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • EJBPS NEW ISSUE PUBLISHED

    Its Our pleasure to inform you that, EJBPS December 2017 Issue has been Published, Kindly check it on http://www.ejbps.com/ejbps/current_issues

  • DECEMBER ISSUE PUBLISHED

    DECEMBER 2017 Issue has been successfully launched on 1 December 2017

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 3.881 to 4.382 due to high quality Publication at International Level.

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT