Abstract
ANTIOXIDANT AND FREE RADICAL SCAVENGER EFFECTS OF METHANOL LEAF EXTRACT OF LUPINUS ARBOREUS.

*Ohadoma S. C. and Eban L. K.

ABSTRACT

Objective: To investigate the antioxidant and free radical scavenger profiles of the leaf extract of Lupinus arboreus. Methods: The crude methanol extract (CME) of the dried leaves obtained by cold maceration for 72 h were analyzed by the methods of 1,1-diphenyl–2–picrylhydrazyl (DPPH), phosphomolybdenum reduction assay, metal ion chelating activity, reducing power assay as well as superoxide radical scavenging assay. Results: The methanol extract of L. arboreus recorded IC50 value of 37.50 μg/ml for DPPH assay; Phosphomolybdenum value was 525± 40.28 mg ascorbic acid equivalent (AAE)/g extract; chelating ability was 35.33± 1.05 mg/EDTA/g extract. The reducing power at varying concentrations (50 – 250) μg/ml were 0.070 to 0.310 absorbance at 700 nm. The superoxide radical scavenging assay showed the IC50 value of 235.05 ± 3.55 μg/ml. Conclusion: The extract of L. arboreus possessed therapeutic value for natural antioxidant and free radical scavenging effects.

Keywords: Lupinus arboreus, antioxidant, free radicals, DPPH, AAE.


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