Abstract
A STUDY ON INVITRO ANTI-DIABETIC ACTIVITY OF CRUDE ACETONE EXTRACT OF RHIZOPHUS SPECIES BY ALPHA AMYLASE INHIBITORY ASSAY.

Pavithra K.* and R. Ushasri

ABSTRACT

Diabetes mellitus is a heterogeneous group of diseases characterized by choric hyperglycaemia with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action, or both. Alpha amylase inhibitors play major role in the management of postprandial hyperglycemia. The aim of the current study was to study on invitro anti-diabetic activity of acetone extract of rhizophus species by alpha amylase inhibitory assay. Rhizopus sps was cultivated on Sabourds agar medium and inoculated in to Sabourds broth and subjected to fermentation at room température for one week.The thick mycelium was seperated by filtration and extracted with Acetone solvent to obtain crude extract. The cytotoxicity of crude extract was determined using INS- 1 cells by MTT assay and were cultured in Rose Well Park Memorial Institute medium. The non cytotoxic concentration of the crude extract was used for antidiabetic activity by alpha amylase inhibition assay. The percentage of alpha-amylase inhibition at different concentration of crude acetone extract of Rhizopus species was found to be (200μg/ml) 24.29%. (250μg/ml) 35.08, %. (300μg/ml) 36.34, %. (350μg/ml) 38.84%., (400μg/ml) 41.84, %. (450μg/ml) 43.84.%.

Keywords: Diabetes, SDA, DNS, Rhizopus, MTT, RPMI, DMSO, INS-1 Alpha amylase.


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