Abstract
SYNERGISTIC ANTIBACTERIAL AND ANTIBIOFILM ACTIVITY OF CHEMICALLY SYNTHESIZED SILVER NANOPARTICLES AGAINST PSEUDOMONAS AERUGINOSA

N. Krithiga and A. Jayachitra*

ABSTRACT

The biofilm are complex structures which undergoes various stages of transformation to form the mature biofilm. The bacteria inside the mature stage are capable to be the causative agent for various infections. In order to eradicate and prevent the biofilm on medical devices implants nowdays the silver nanoparticles are utilized. The new antibiofilm technologies target various stages of biofilm formation silver nanoparticles possess potential antibacterial and antibiofilm properties. The silver nanoparticles characterized by spectral analysis where the surface Plasmon resonance at 420 nm, XRD average size 26.5nm, the SEM & TEM micrographs reveals the nature of the silver nanoparticles to be spherical in nature. The antibacterial activity of silver nanoparticles showed the potential activity. The visualization of the antibiofilm was observed with the help of optical microscope, crystal violet staining, titre plate method, congo red agar plate and SEM micrographs. From all the observed results it was proved that the silver nanoparticles would be the powerful weapon to eradicate the biofilm over the surgical instruments.

Keywords: Silver nanoparticles, XRD, antibacterial, antibiofilm effect, SEM.


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