Abstract
EXTRACTION AND CHROMATOGRAPHIC ANALYSIS OF ?- CAROTENE PRODUCED BY RED YEASTS

M. Dhaliwal* and N. Chandra

ABSTRACT

Carotenoid diversity is important because of both its biotechnological potential and its role in understanding the evolution of secondary metabolism. The present study involves chromatographic profiling of the pigment extracts of the 14 wild type red yeast isolates in comparison with type culture Rhodotorula glutinis NCIM 3353. The HPTLC and HPLC analysis revealed presence of β -carotene in most of the yeast isolates. The chromatogram of the 14 wild type isolates along with the type strain Rhodotorula glutinis NCIM 3353 was scanned at 430 nm for separated bands of various carotenoids present in the pigment extracts. The approximate concentration of β-carotene in the type strain Rhodotorula glutinis NCIM 3353 was calculated to be 35.11μg ml-1, amounting to 69% of the total carotenoids present in the sample. From the chromatograms higher concentration of β-carotene (219.5 μg ml-1) was found in the pigment extract of soil isolate S1, Rhodotorula mucilaginosa MTCC 11823.

Keywords: Carotenoids; chromatographic profile; ?- carotene; Rhodotorula mucilaginosa.


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