European Journal of Biomedical and Pharmaceutical Sciences

COMPARATIVE ESTIMATION OF PLATELETS CYTOKINES WITH AND WITHOUT ADDITIVE SOLUTION

Dr. Subhashish Das* and Dr. Harendra Kumar M. L.

ABSTRACT

Background: Despite continuous efforts to improve the process and in spite of major advances in establishing optimized storage conditions, platelet storage lesions still occur. These are characterized by loss of function and changes in morphophysiological characteristics associated with platelet activation, vacuolization, microvesiculation and fragmentation. A poor quality of platelets may lead to a decrease in recovery after transfusions and shorter survival times of circulating platelets. Objective: The aim of this study was to compare the various cytokines levels in the stored platelets. Methods: Units of whole blood were drawn using a quadruplebag blood container system. Platelet rich plasma prepared from whole blood following standard methods was stored for 10 days. During this period test samples were aseptically collected for analysis on Days 1, 2, 3, 5, 7 and 10. Results: There was a significant increase in the mean IL-1β and TNF after 10 days of storage in platelets without additive solutions as compared to the platelets stored in additive solution. Conclusions: This study shows that IL-8 levels significantly increased after 1 h of storage and TNF-α levels were low up to 18 h and then showed increase but not as marked as IL-8. This study highlights the time dependent release of IL-8 and TNF-αin PC during 5 days of storage at 22ᵒC.

Keywords: Platelet-rich plasma; Cytokines; Platelet Additive Solution.