COMPARATIVE ESTIMATION OF PLATELETS CYTOKINES WITH AND WITHOUT ADDITIVE SOLUTION
Dr. Subhashish Das* and Dr. Harendra Kumar M. L.
ABSTRACT
Background: Despite continuous efforts to improve the process and in spite of major advances in establishing
optimized storage conditions, platelet storage lesions still occur. These are characterized by loss of function and
changes in morphophysiological characteristics associated with platelet activation, vacuolization,
microvesiculation and fragmentation. A poor quality of platelets may lead to a decrease in recovery after
transfusions and shorter survival times of circulating platelets. Objective: The aim of this study was to compare
the various cytokines levels in the stored platelets. Methods: Units of whole blood were drawn using a quadruplebag
blood container system. Platelet rich plasma prepared from whole blood following standard methods was
stored for 10 days. During this period test samples were aseptically collected for analysis on Days 1, 2, 3, 5, 7 and
10. Results: There was a significant increase in the mean IL-1β and TNF after 10 days of storage in platelets
without additive solutions as compared to the platelets stored in additive solution. Conclusions: This study shows
that IL-8 levels significantly increased after 1 h of storage and TNF-α levels were low up to 18 h and then showed
increase but not as marked as IL-8. This study highlights the time dependent release of IL-8 and TNF-αin PC
during 5 days of storage at 22ᵒC.
Keywords: Platelet-rich plasma; Cytokines; Platelet Additive Solution.
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