Abstract
GENOME ELIMINATION MEDIATED POLLEN SIZE DIMORPHISM IN CLONE OF SACCHARUM SPONTANEUM L. (POACEAE): A PATHWAY TO INTRASPECIFIC POLYPLOIDY

Chandra Bhanu Singh, Vijay Kumar Singhal* and Rohit Kumar

ABSTRACT

Background: Saccharum spontaneum is the most valuable germplasm in sugarcane breeding. The paper aims to unravel the mechanism of genome elimination mediated pollen size dimorphism and its possible role in the evolution of polyploid series of a basic set of eight chromosomes (x = 8) in the species under study. Methods: The spikelets were collected from the clone growing under normal and submerged conditions were fixed separately, subsequently transferred in the fixative and stored in refrigerator at 4ยบ C until use. Meiocytes preparations were made by using standard acetocarmine procedure and were examined at different stages of meiosis. Mature anthers were taken for pollen grain study. Results and Discussion: The pollen mother cells (PMCs) of a male sterile octoploid (2n=8x=64) clone of species formed 32 bivalents at prophase-I and 32 or 24 bivalents at M-I. The PMCs containing 24 bivalents emerged from those anthers which were flooded for over six continuous hot sunny days. The bivalent arrangements into two and more dissimilar groups in derived PMCs seemed to be the further steps of genome elimination. Consequently, anaphase I segregation figured as 16:16 and 24:24 in flood stressed PMCs whereas only 32: 32 in normal ones and formation of large pollen grains in approximate frequency denoted their respective ploidy level as 2x and 3x for evolution of polyploids. Conclusion: The genome elimination mediated pollen size dimorphism seems to have played a significant role in the formation of polyploid series of a genome comprised of 8 chromosomes (x=8) in S. spontaneum.

Keywords: Genome elimination, Pollen dimorphism, Polyploidization, Saccharum, Pollen mother cell, meiosis.


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