Abstract
GC-MS ANALYSIS, PHYTOCHEMICAL, AND ANTIMICROBIAL ACTIVITY OF SUDANESE NIGELLA SATIVA (L) OIL

*Tuhami E. Hagr, Kauther S. Ali, Amira A. E. Satti and Omer S. A.

ABSTRACT

Preliminary phytochemical screening of ethanolic crude extract from Nigella Sativa seeds Indicated the presence of Alkaloids, Flavonoid, Carbohydrate, SaponinsTriterpen, Tannins, phenolic and Steroids. Maceration extraction of Nigella Sativa (L) seeds was extract with n-hexane gave 13 ml of crude oil. Eighteen components were detected by GC-MS analysis. Major constituents are Hexadecanoic acid, methyl ester (1.79%), n-Hexadecanoic acid (7.97% ,9, 12-Octadecadienoic acid (Z,Z) -, methyl ester (8.43 %),9-Octadecanoic acid (Z)-,methyl ester (3.48%), 9,12-Octadecadienoic acid (Z,Z)methyl ester (48.72%), Oleic acid (13.14 %), Heptadecanoic acid (1.59),9,12-Octadecadienoyl chloride (Z,Z) (8.10%), Methyl 2-hydroxy-octadeca-9,12,15-trienote (3.53%). The oil Nigella Sativa (L) seeds showed moderately activity against organisms.

Keywords: GC-MS Analysis, Phytochemical, and Antibacterial activity.


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