Fatima A. I. Younis, Hasabo A. M. Ahmed*, Fragon M. Ahmed, Ramadan M. Awad and Magdi Gibril


In the present research, cost effective and environmentally friendly gold nano particles (AuNPs) were synthesized. Biosynthesis of AuNPs was carried out using the aqueous extract of fenugreek seeds (Trigonellafoenum-graecum) as reducing and capping agent. The principle is based on the reduction of Aucl4 by the extract of fenugreek seeds. Gold nanoparticles having different sizes in the range from 15 to 25 nm were obtained by controlling the synthesis parameters. Physico-chemical characteristics were carried out by UV-Vis spectra, fluorescent activity, dynamic light scattering and transmission electron microscopy. UV-Vis spectra showed the maximum absorbance of GNP at 535 nm. Transmission electron microscopy (SEM) showed that the shape of GNP was nearly spherical with 5–20 nm size. After addition of plant extract in the gold salt solution, the color changed from pale yellow to ruby red and deep purple indicating the formation of AuNPs. This is due to the synthesis of AuNPs with the molecular assistance of biological reducing agents represented by the fenugreek seeds extract. The appearance of the color varied drastically with the temperatures, and the amount of extract at 50ºC, more time was required for the complete reduction; whereas at 100ºC the color appeared in few mints. The clear colloidal suspension of AuNPs was stable for more than two months at 4ºC. The corresponding gold nanoparticles solutions contained in glass showed the variations in color of AuNPs due to excitation of surface Plasmon vibrations. The AuNPs is intended for further investigations in the area of drugs in regard to toxicity.

Keywords: Biosynthesis, Gold nanoparticles, Plant Extract, Surface Plasmon.

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