Abstract
THE STUDY OF THE ANTIMICROBIAL PROPERTY OF FIXED OIL FROM THE DIFFERENT SPECIES OF CUCURBITACEAE FAMILY

A. F. O. Cabras, *J. P. Delfin, J. L. Juanico, C. M. A. Marin, S. M. S. Montero, Mylene S. Andal, RPh, MS Pharm Susan S. Montemayor, RPh, MS Pharm. Pharm.D

ABSTRACT

This research presents the results of which among the three species of Cucurbitaceae Family namely Squash (Cucurbita maxima), Luff (Luffa acutangula), and Cucumber (Cucumis sativus) exhibit antimicrobial property. There are different tests conducted like the physical test, determining the color, odor and appearance of the three plant sample oil. The solubility test using 0.5 ml of the following solvents: water, alcohol, ether, chloroform, and acetone. Squash (Cucurbita maxima) is highly immiscible in all solvents used. Cucumber (Cucumis sativus), two of the solvents used is miscible with ether and chloroform while immiscible with distilled water, 95% ethanol and acetone while Luff (Luffa acutangula) is immiscible with the distilled water, 95% ethanol, ether and miscible with chloroform and acetone. Percentage yield was also determined, percentage yield of the extracted fixed oils of Luff (Luffa acutangula) was 46.93%, Squash (Cucurbita maxima) 35.80% and Cucumber (Cucumis sativus) 31.36%. With the stain test, all of the oil from the seed of squash, cucumber and Luff give permanent stain on the paper. The result obtained in specific gravity of the Fixed oils from luffa was 0.67, Cucumber 0.67 and Squash 0.69. Three concentrations of the fixed oil extract (250mg/ml, 500mg/ml, and 1000mg/ml) were used in this study to conduct the microbial assay of the fixed oil extract. The collected concentrations of the fixed oil extract were used for the anti-microbial property assay against staphylococcus aureus for gram positive bacteria, Escherichia coli for gram negative bacteria and candida albicans for fungi. Agar well diffusion method was used in this study using 45 plates for the microbial assay of the fixed oil. The researchers used ciprofloxacin as the positive and negative control for gram positive and gram negative bacteria, and fluconazole as the positive control for fungi. Sterile water was used as the negative control in this study. Based on the result of the study, the researchers concluded that the extracted fixed oil from the seeds of cucumber, squash and Luff did not exhibit any antimicrobial property.

Keywords: Cucurbitaceae Family, Antimicrobials, Fixed oil, seeds oil.


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