Abstract
BIOTECHNOLOGICAL APPROACH OF PHYTOPATHOGENIC ORGANISMS (LYSTERIA MONOCYTOGENES) FROM THE LEAF IN VIGNA RADIATA (L.)

Divya V. and S. Sujatha*

ABSTRACT

V. radiata (L.) is a legume cultivated for its edible seeds and sprouts across Asia. The present research work was aimed to identify the dominant autochthonous bacterial flora from the leaf blight disease causative agent on the experimental host plant of V. radiata (L.). Totally four different organisms were isolated and the dominant bacterial isolatesc were confirmed as “Lysteria monocytogenes” through sequence analysis. Proteins are the major biological factor that plays an important role in the bacterial development and various physiological processes. Consequently, the protein profile of the phytopathogenic bacterial strains especially L. monocytogenes has been identified its proteins polypeptides in the form of banding pattern throuha technique of SDS-PAGE. Interestingly PPB3 (Lysteria monocytogenes) bacteria expressed the total of six bands, of these six one band was almost prominently thickened appearance than the other five derived bands. In addition these bacteria possessed the highest and lowest molecular weight range between 279 to 162 kDa protein band intensity. From the overall result, interestingly it was revealed that the determined protein SDS banding pattern of disease causative agents. This was the first recordically reported work for assessed the protein profiling of the disease causative organism of L. monocytogenes from the legume plant especially leaf of V. radiata (L.) plant. This is the first recordically reported work for assessing the protein profile of the disease causative organism from the legume plant especially leaf of V. radiata (L.) plant.

Keywords: V. radiata, leaf blight disease, sequence analysis, SDS-PAGE.


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