Abstract
161 A REVIEW ON HPLC COLUMN CHARACTERISTICS AND SPECIFICATION

Deelip Derle and Archana S. Chavan*

ABSTRACT

The column is an two types reversed phase and normal phase column. The column packed with different particle size of stationary phases. It often an efficiency of column. E.g. 5μm porous particle size is best suited for number of solutes. The small particle size(3սm, 5սm), longer length(25cm) of column shows more number of theoretical plates, plate number more than 2000 ie, more efficient column. For column packing mostly silica use because its high mechanical strength. In bonded phase the sterically protected organic groups attached to silane group gives less concentration of silane group shows more efficiency of bonded phase. Peak asymmetry depend on column particle size, equal sphericle size particle gives asymmetry value less than 1.5.

Keywords: Column, HPLC, triethylamine, porous particle, theoretical plate number, bonded phase silica.


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