Abstract
A STUDY OF METHOD DEVELOPMENT, VALIDATION AND FORCED DEGRADATION FOR SIMULTANEOUS QUANTIFICATION OF GLECAPREVIR AND PIBRENTASVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Dr. G. Alluraiah*, G. Kishore, Dr. Y. Hanumantha Rao and P. Srinivasa Rao

ABSTRACT

Objective: The present paper describes a simple, accurate and precise reversed phase HPLC method for rapid and simultaneous quantification of Glecaprevir and Pibrentasvir in bulk and pharmaceutical dosage form. The chromatographic separation was achieved on Luna C18 (150x4.6mm, 3.5μm). Mobile phase contained a mixture of 0.1% Ortho Phosphoric acid (OPA) and Acetonitrile in the ratio of 50:50 v/v, flow rate 1.0ml/min and UV detection at 258nm. The proposed method shows a good linearity in the concentration range of 10-150 μg/ml for Glecaprevir and 4-60 μg/ml for Pibrentasvir under optimised conditions. Precision and recovery study results are in between 98-102%. In the entire robustness conditions %RSD is below 2.0%. Degradation has minimum effect in stress condition and solutions are stable up to 24 hrs.

Keywords: Glecaprevir and Pibrentasvir.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • NEW MARCH ISSUE PUBLISHED

    MARCH 2019 Issue has been successfully launched on 1 March 2019

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 4.382 to 4.918 due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT