Abstract
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF NEW ANTICANCER AGENT ACALABRUTINIB IN BULK AND ITS PHARMACEUTICAL FORMULATION

Priyanka P., Shyamala*, Mounika. D, Nadeemuddin and Abdul Moyeez

ABSTRACT

A novel isocratic reverse phase liquid chromatography method for determination of Acalabrutinib was developed and validated after optimization of various chromatographic conditions.Chromatographic conditions used are stationary phase kromosilC18 column (250×4.6 mm5m) with mobile phase 0.1% O-Phosphoric acid and methanol in ratio of 50:50 and flow rate was maintained at 1.0 ml/min, detection wavelength was 236nm,column temperature was set to 300C and diluents was mobile phase conditions were finalized as optimized method.System suitability parameters were studied by injecting the standard six times and results were under acceptance criteria. Linearity study was carried out between 25% to 150% levels, R value was found to be 0.4 for repeatability and 0.8for intermediate precision.LOD and LOQ are 0.73μg/ml respectively. The retention time of Acalabrutinib was 2.6min. The linearity for Acalabrutinib was in the range of 25-150 μg mL-1 with coefficient of correlation 0.999. The proposed method was validated with respect to linearity, accuracy, precision and robustness.

Keywords: Acalabrutinib, RP-HPLC, Validation.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • NEW APRIL ISSUE PUBLISHED

    APRIL 2019 Issue has been successfully launched on 1 April 2019

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 4.382 to 4.918 due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT